[CAS NO. 6451-73-6] Scoulerine
PRODUCTS SPECIFICATIONS [6451-73-6]
DESCRIPTION [6451-73-6]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 327.37 |
| Molecular Formula | C19H21NO4 |
| SMILES | OC1=C(OC)C=C(CCN2CC3=C(O)C(OC)=CC=C3C[C@]24[H])C4=C1 |
Summary
In Vitro
Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC
50
s ranging from 2.7 µM to 6.5 µM
[1]
.
Scoulerine (2.5-20 µM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells
[1]
.
Scoulerine (2.5-20 µM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis
[1]
.
Scoulerine induces G2 or M cell cycle arrest
[1]
.
Scoulerine (2.5-5 µM; 24 hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells
[1]
.
Scoulerine (2.5-5 µM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner
[1]
.
Scoulerine (5-10 µM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells
[1]
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | Jurkat and MOLT-4 cells |
| Concentration: | 2.5, 5, 10, 15 and 20 µM |
| Incubation Time: | 24 hours |
| Result: | Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner. |
Apoptosis Analysis [1]
| Cell Line: | MOLT-4 and Jurkat cells |
| Concentration: | 2.5, 5, 10, 15 and 20 µM |
| Incubation Time: | 24 hours |
| Result: | Induced MOLT-4 and Jurkat cells apoptosis. |
Cell Cycle Analysis [1]
| Cell Line: | Jurkat and MOLT-4 leukemic cells |
| Concentration: | 2.5-20 µM |
| Incubation Time: | 16 hours |
| Result: | Induced cell cycle arrest at the G2/M transition. |
Western Blot Analysis [1]
| Cell Line: | MOLT-4 cells |
| Concentration: | 2.5, 5 µM |
| Incubation Time: | 24 hours |
| Result: | Showed an upregulation of p53 protein in p53 wild-type MOLT-4 cells. |
Appearance
Solid
Source
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL ( 305.46 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.0546 mL | 15.2732 mL | 30.5465 mL |
| 5 mM | 0.6109 mL | 3.0546 mL | 6.1093 mL |
| 10 mM | 0.3055 mL | 1.5273 mL | 3.0546 mL |
Synonyms
