[CAS NO. 36455-72-8] α-Zearalenol
PRODUCTS SPECIFICATIONS [36455-72-8]
DESCRIPTION [36455-72-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 320.38 |
| Molecular Formula | C18H24O5 |
| SMILES | OC1=C2C(O[C@H](CCC[C@@H](CCC/C=C/C2=CC(O)=C1)O)C)=O |
Summary
In Vitro
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) shows the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells with an IC
50
of 12.5 μM
[1]
.
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) induces an increase of cell viability on MCF-7 cells mainly after 72 h of treatment whereas it is slightly cytotoxic on MDA-MB231 cells
[2]
.
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) increases MCF7 cell population in the S phase and in the G2/M phase, no visible change in cell cycle phases is observed in MDA-MB231 cells
[2]
.
α-Zearalenol (α-zol) (1-10 μM; 24 hours) reduces the expression of TNF-α, α-ZOL combines with β-ZOL shows an antagonistic effect on inflammation for IL-1β and act synergic for IL-8 at high toxin concentrations
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | MFC-7 cells |
| Concentration: | 6.25 µM-25 µM |
| Incubation Time: | 24 hours |
| Result: | Observed the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells. |
Cell Viability Assay [2]
| Cell Line: | Breast cancer cell lines: MCF-7 and MDA-MB231 |
| Concentration: | 0.001 μM, 0.1 μM and 10 μM |
| Incubation Time: | 24 hours, 48 hours, 72 hours |
| Result: | Modified the cell growth in two breast cancer cell lines. |
Cell Cycle Analysis [2]
| Cell Line: | MCF7 cells, MDA-MB231 cells |
| Concentration: | 0.001 μM, 0.1 μM and 10 μM |
| Incubation Time: | 72 hours |
| Result: | Observed an increase of cells in cell cycle in MCF7 cells. |
RT-PCR [2]
| Cell Line: | HepG2 cells |
| Concentration: | 1 μM, 5 μM, 10 μM |
| Incubation Time: | 72 hours |
| Result: | Induced a decrease of the expression of TNF-α, IL-1β and IL-8, that was in most cases statistically significant for the higher doses. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 312.13 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.1213 mL | 15.6065 mL | 31.2129 mL |
| 5 mM | 0.6243 mL | 3.1213 mL | 6.2426 mL |
| 10 mM | 0.3121 mL | 1.5606 mL | 3.1213 mL |
References
- [1] .Tatay E, et al. Estrogenic activity of zearalenone, α-zearalenol and β-zearalenol assessed using the E-screen assay in MCF-7 cells. Toxicol Mech Methods. 2018 May;28(4):239-242.
- [2] Marin DE, et al. Cytotoxic and inflammatory effects of individual and combined exposure of HepG2 cells to zearalenone and its metabolites. Naunyn Schmiedebergs Arch Pharmacol. 2019 Aug;392(8):937-947.
- [3] Nittoli AC, et al. Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach. Metabolomics. 2018 Feb 14;14(3):33.
Synonyms
