[CAS NO. 328-50-7] 2-Ketoglutaric acid
PRODUCTS SPECIFICATIONS [328-50-7]
DESCRIPTION [328-50-7]
Overview
| MDL | MFCD00004165 |
|---|---|
| Molecular Weight | 146.10 |
| Molecular Formula | C5H6O5 |
| SMILES | OC(=O)CCC(=O)C(O)=O |
Summary
2-Ketoglutaric acid (Alpha-Ketoglutaric acid) is an intermediate in the production of ATP or GTP in the Krebs cycle. 2-Ketoglutaric acid also acts as the major carbon skeleton for nitrogen-assimilatory reactions. 2-Ketoglutaric acid is a reversible inhibitor of tyrosinase ( IC 50 =15 mM) [1] .
IC50 & Target
|
Human Endogenous Metabolite
|
Microbial Metabolite
|
Tyrosinase 15 mM (IC 50 ) |
In Vitro
2-Ketoglutaric acid (Alpha-Ketoglutaric acid) has other physiological capabilities including reduction of ammonia level formed in the lung and general ammonia detoxification, protective role against lipid peroxidation and neuroprotective effect against cyanide poisoning
[1]
.
2-Ketoglutaric acid acts as precursor for the synthesis of amino acids and nucleotides
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 250 mg/mL ( 1711.16 mM ; Need ultrasonic)
H 2 O : 50 mg/mL ( 342.23 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 6.8446 mL | 34.2231 mL | 68.4463 mL |
| 5 mM | 1.3689 mL | 6.8446 mL | 13.6893 mL |
| 10 mM | 0.6845 mL | 3.4223 mL | 6.8446 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (684.46 mM); Clear solution; Need ultrasonic
-
2.
-
3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (14.24 mM); Clear solution
-
4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (14.24 mM); Clear solution
References
- [1] Huergo LF, et al. The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite. Microbiol Mol Biol Rev. 2015 Dec;79(4):419-35.
- [2] Gou L, et al. The effect of alpha-ketoglutaric acid on tyrosinase activity and conformation: Kinetics and molecular dynamics simulation study. Int J Biol Macromol. 2017 Dec;105(Pt 3):1654-1662.
Synonyms
